View Full Version : Olive polyphenols - what dose is required to improve insulin sensitivity?

08-03-2009, 02:43 PM
I'm having a tough time finding out exactly how much EVOO or actual olives are required to get enough of the polyphenols to improve insulin function. I mean it's tough to make a diet for this when you don't know if 1 tsp of EVOO or 3 tbsp is needed to get an effect. Of course levels would vary in olives and olive oils, but is there some general idea of what's needed? Tough time finding any literature on this.

08-03-2009, 10:53 PM
have you tryed the olive leaf extract from the herb shop?

08-04-2009, 09:14 PM
as far as i know, the studies have yet to provide us with any exact dosages of monounsaturated fats that we could apply. also, all the studies on the mediterranean diet dont quantify the amount of olive oil.

the closest thing i know of is the international guidelines for the nutritional management and prevention of type 2 diabetes mellitus. they recommend 10-20 percent of monos but, they also recommend 45-60 percent of carbs. so take that for whatever it is.

does anyone have any sources to the original question?

11-17-2009, 01:57 AM
have you tryed the olive leaf extract from the herb shop?

Olive leaf extract oleuropein Is an extremely potent anti-viral/anti-bacterial agent. When you take it it makes you feel very sick and lethargic. I have no idea why anyone would incorporate this compound into their regiment unless suffering from the flu, or something similar.

As far as EVOO, I am not aware of any studies suggesting optimal dosages?

I take 2 tablespoons/day, and use it for cooking. But I use a literal shit ton of fish oils as well, so even anectdotely I can't say I see any positive effects?

11-17-2009, 12:57 PM
J Nutr Biochem. (javascript:AL_get(this,%20'jour',%20'J%20Nutr%20B iochem.');) 2009 Jul 17. [Epub ahead of print]
Oleuropein aglycon prevents cytotoxic amyloid aggregation of human amylin.

Rigacci S (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Rigacci%20S%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Guidotti V (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Guidotti%20V%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Bucciantini M (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Bucciantini%20M%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Parri M (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Parri%20M%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Nediani C (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Nediani%20C%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Cerbai E (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Cerbai%20E%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Stefani M (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Stefani%20M%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Berti A (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Berti%20A%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract).
Department of Biochemical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
Pancreatic amyloid deposits of amylin are a hallmark of Type II diabetes and considerable evidence indicates that amylin oligomers are cytotoxic to beta-cells. Many efforts are presently spent to find out naturally occurring molecules, or to design synthetic ones, able to hinder amylin aggregation or to protect cells against aggregate cytotoxicity. In this context, a protective effect of some polyphenols against amyloid cytotoxicity was reported. Actually dietary polyphenols are endowed with multiple health benefits, and extra virgin olive oil is attracting increasing interest as a source of these substances. Here, we investigated the effects on amylin aggregation and cytotoxicity of the secoiridoid oleuropein aglycon, the main phenolic component of extra virgin olive oil. We found that oleuropein, when present during the aggregation of amylin, consistently prevented its cytotoxicity to RIN-5F pancreatic beta-cells, as determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide test and caspase-3 activity assay. A lack of interaction with the cell membrane of amylin aggregates grown in the presence of oleuropein was shown by fluorescence microscopy and synthetic lipid vesicle permeabilization. Moreover, our ThT assay, circular dichroism analysis and electron microscopy images suggested that oleuropein interferes with amylin aggregation, resulting in a different path skipping the formation of toxic pre-fibrillar aggregates. These results provide a molecular basis for some of the benefits potentially coming from extra virgin olive oil consumption and pave the way to further studies on the possible pharmacological use of oleuropein to prevent or to slow down the progression of type II diabetes.

PMID: 19616928 [PubMed - as supplied by publisher]

Interesting. I was actually unaware oleuropein was present in olive oil. Frosty, this may be one possibility of it MOA.

11-17-2009, 02:09 PM
Planta Med. (javascript:AL_get(this,%20'jour',%20'Planta%20Med .');) 2009 Aug;75(10):1141-3. Epub 2009 Mar 16.
Effects of maslinic acid, a natural triterpene, on glycogen metabolism in cultured cortical astrocytes.

Guan T (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Guan%20T%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Li Y (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Li%20Y%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Sun H (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Sun%20H%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Tang X (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Tang%20X%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Qian Y (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Qian%20Y%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract).
Department of Physiology, China Pharmaceutical University, Nanjing, P.R. China
Maslinic acid (2- alpha,3- beta-dihydroxyolean-12-en-28-oic acid) is a triterpenoid compound present in plants of Olea europaea. In the present study, we investigated the effect of maslinic acid on astrocytic glycogen metabolism. Glycogen phosphorylase (GP) activity in homogenates of cultured astrocytes was analyzed, and maslinic acid exhibited GP inhibition with an IC (50) value of 5.7 microM. Moreover, the influence of maslinic acid on glycogen synthesis and glycogenolysis was also investigated. Pre-incubation with maslinic acid dose-dependently increased cellular glycogen content and prevented the excessive glycogenolysis induced by norepinephrine. In conclusion, maslinic acid is suggested to be a potent inhibitor of astrocytic glycogen phosphorylase. Georg Thieme Verlag KG Stuttgart.New York.

PMID: 19291614 [PubMed - indexed for MEDLINE]

11-17-2009, 02:10 PM
Phytother Res. (javascript:AL_get(this,%20'jour',%20'Phytother%20 Res.');) 2009 Mar;23(3):347-50.
Antidiabetic effect of Olea europaea L. in normal and diabetic rats.

Eidi A (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Eidi%20A%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Eidi M (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Eidi%20M%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Darzi R (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Darzi%20R%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract).
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. [email protected]
The antidiabetic effect of an alcohol extract of olive (Olea europaea L.) leaves was investigated in normal and streptozotocin-induced diabetic rats. The oral administration of the olive leaves extract (0.1, 0.25 and 0.5 g/kg body wt) for 14 days significantly decreased the serum glucose, total cholesterol, triglycerides, urea, uric acid, creatinine, aspartate amino transferase (AST) and alanine amino transferase (ALT) while it increased the serum insulin in diabetic rats but not in normal rats (p < 0.05). A comparison was made between the action of olive leaves extract and glibenclamide (600 microg/kg), a known antidiabetic drug. The antidiabetic effect of the extract was more effective than that observed with glibenclamide. (c) 2008 John Wiley & Sons, Ltd.

PMID: 18844257 [PubMed - indexed for MEDLINE]

11-17-2009, 02:17 PM
Biochem Biophys Res Commun. (javascript:AL_get(this,%20'jour',%20'Biochem%20Bi ophys%20Res%20Commun.');) 2007 Nov 3;362(4):793-8. Epub 2007 Jul 3.
Anti-hyperglycemic activity of a TGR5 agonist isolated from Olea europaea.

Sato H (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Sato%20H%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Genet C (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Genet%20C%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Strehle A (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Strehle%20A%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Thomas C (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Thomas%20C%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Lobstein A (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Lobstein%20A%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Wagner A (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Wagner%20A%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Mioskowski C (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Mioskowski%20C%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Auwerx J (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Auwerx%20J%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Saladin R (http://www.ncbi.nlm.nih.gov/pubmed?term=%22Saladin%20R%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract).
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/Université Louis Pasteur, 67404 Illkirch, France.
Olive tree (Olea europeaea) leaves are well known for their effect on metabolism in particular as a traditional anti-diabetic and anti-hypertensive herbal drug. These properties are until now only attributed to oleuropein, the major secoiridoid of olive leaves. Here we describe the isolation and the identification of another constituent implicated in the anti-diabetic effect of this plant, i.e. oleanolic acid. We show that this triterpene is an agonist for TGR5, a member of G-protein coupled receptor activated by bile acids and which mediates some of their various cellular and physiological effect. Oleanolic acid lowers serum glucose and insulin levels in mice fed with a high fat diet and it enhances glucose tolerance. Our data suggest that both oleuropein and oleanolic acid are involved in the anti-diabetic effect of olive leaves and further emphasize the potential role of TGR5 agonists to improve metabolic disorders.

PMID: 17825251 [PubMed - indexed for MEDLINE]

11-17-2009, 02:27 PM
Possible mirror action of 1,5-Anhydroxylitol, and 1,5-Anhydroglucitol?

This may be a contributing factor in the effects of olive leaf and byproducts on the bloodglucose system?

Might be wrong here, but these two compounds are very similar. and 1,5-Anhydroglucitol is used to determine glycemic variability in diabetics. Possible link, maybe???